Sample was taken from beach simulator tank in marine biotechnology, kamaishi, iwateken, japan on november 2005. Isolation of hydrocarbon degrading bacteria from soils. Source of isolation the source for bacterial isolation consisted of wastewaters contaminated with. The bacterial strains were isolated by inoculating the soil and water sample into the prepared nutrient agar medium which is autoclaved and added 1. No correlation was found between the prevalence of hydrocarbondegradative genotypes and biological activities respiration, fluorescein diacetate hydrolysis, lipase activity or numbers of culturable hydrocarbondegrading soil microorganisms. Petroleum hydrocarbondegrading bacteria for the remediation of. A large number of degrading bacteria from contaminated cold soils have been identified, including representatives of gramnegative and grampositive genera 1, 8, 9, 17, 49, 50. Bacteria are the most active agents in petroleum degradation. Floodgate 12 listed 25 genera of hydrocarbon degrading bacteria and 25 genera of hydrocarbon degrading fungi, which were mukherjee an overview on microbial degradation of petroleum hydrocarbon contaminants jahangeer and vikram kumar. This study was aimed at bacterial degradation of polycyclic aromatic hydrocarbons pah in untreated refinery effluent. Abstract many hydrocarboncontaminated environments are characterized by low or elevated temperatures, acidic or alkaline ph, high salt concentrations, or high pressure.
Isolation and characterization of hydrocarbon degrading bacteria from environmental habitats in western new york state, masters thesis in chemistry, 2005. Twentythree hydrocarbondegrading bacteria strains were isolated from gas station leakingcontaminated groundwater. Hydrocarbondegrading microorganisms, adapted to grow and thrive in these environments, play an important role in the biological treatment of polluted extreme habi tats. Bacteria are the most active agents in petroleum degradation, and they work as primary degraders of spilled oil in environment 37, 38. The occurrence of polycyclic aromatic hydrocarbons pah in refinery effluents is of great concern globally due to its persistence, recalcitrance and carcinogenicity. Pdf with the sharp increase in population and modernization of society, environmental pollution resulting from petroleum hydrocarbons has. The bacteria can also through the production of biosurfactant and conversion of heavy oil compounds to lighter compounds and use them as.
A total of 1 strains of hydrocarbon degrading bacteria were isolated from the surface sediment samples, during the period between july 2009 june 2010, slightly predominated by gramnegative bacteria. Metabolic diversity of aromatic hydrocarbondegrading bacteria from a petroleumcontaminated aquifer mark d. Contaminated soil samples were collected from warri refinery jetty while water sample was collected from. Hydrocarbondegrading bacteria could supply the algae with the benefits outlined in figure 4. Mittal and singh 2009, have reported eleven petroleum hydrocarbon degrading bacterial isolates from oil production site of lingala oil field, ongc and nine isolates from oil contaminated soil of local area at haridwar. Introduction the biodegradation of petroleum and other hydrocarbons in the environment is a. For these facultative hydrocarbon degraders the occasional supertanker oil spill forms an occasional carbon banquet. Hydrocarbon degrading bacteria in oily sludge through the decomposition of these compounds reduce the waste volumes and are able to reduce their toxicity. Pdf microbial degradation of petroleum hydrocarbons. Floodgate 36 listed 25 genera of hydrocarbon degrading bacteria and 25 genera of hydrocarbon degrading fungi which were isolated. Isolation and characterization of hydrocarbondegrading bacteria.
Mix population of these microbes are more efficient. Isolation and molecular identification of hydrocarbon degrading bacteria from oilcontaminated soil fatiha dilmi 1, 2, abdelwaheb chibani 1, khadidja senouci rezkallah 2 1laboratory of microbiology and plant science, department of biology, faculty of natural and life sciences, university of abdelhamidibn badis, mostaganem, algeria. Several bacteria are even known to feed exclusively on hydrocarbons 39. Isolation and identification of petroleum hydrocarbon. Here, we showed that the isolation and screening strategy affected a lot, the selection of the hydrocarbondegrading bacteria, by combining the.
Characterization of hydrocarbon degrading bacteria isolated from hydrocarbon contaminated soil. Natural mechanism for degrading hcs by bacteria, yeast, and fungi. In the same sand samples for which detailed chemical analysis was performed, mpn counts of cultivatable hydrocarbondegrading bacteria in oilcontaminated sands 2. Isolation, screening and activity of hydrocarbondegrading. Screening and isolation of a hydrocarbon degrading. Biodegradation and bioremediation of hydrocarbons in. Characteristics of aerobic microorganisms capable of degrading organic pollutants 146 2 principles of bacterial degradation 147 2. Analysis of aromatic hydrocarbon degrading capacity by. Pdf hydrocarbondegrading bacteria and the bacterial. Isolation and identification of effective hydrocarbon degrading bacteria from diesel polluted soil doi. Created using powtoon free sign up at create animated videos and animated presentations for free. Characterization of hydrocarbondegrading bacteria isolated from oilcontaminated sediments in the sultanate of oman and evaluation of bioaugmentation and biostimulation approaches in microcosm experiments.
The purpose of this research was to identify hydrocarbon degrading bacteria which grown on phenanthrene, naphthalene, trichlorodibenzofuran and benzoapyrene. Isolation and identification of hydrocarbon degrading. The level of petroleum hydrocarbon degradation was determined by gravimetric assay at each 5 days interval. This confirms that not only do a variety of hydrocarbon degrading bacterial populations exist in the newly created deepsea oil plume, but also, as the authors state that the microbial communities appear to be undergoing rapid dynamic adaptation in response to oil contamination. Isolation, screening and activity of hydrocarbondegrading bacteria. Isolation and characterization of crude oil degrading. Reconstructing metabolic pathways of hydrocarbondegrading bacteria from the deepwater horizon.
Anaerobic biodegradation of saturated and aromatic. Supplied in a dry bran mixture, microorganisms are activated when mixed with water. Since this system was described, alkb homologous have been found in many alkanedegrading. Aerobic degradation of crude oil by microorganisms in soils from. Alcanivorax borkumensis was the first hcb to have its genome sequenced. Comparative studies on bacterial consortia for hydrocarbon. Mexican scientists participate in massive study to identify and cultivate bacteria. Optimization of growth condition for hydrocarbon degrading. Anaerobic degradation under nitratereducing bacteria sulfatereducing bacteria 17. Results the result of the bacterial count showed 38. Isolation and characterization of hydrocarbondegrading. Various methods are used to characterize hydrocarbondegrading populations in soil. Department of microbiology, rishi biotech, mumbai400053, india corresponding author a b s t r a c t experiments were performed in low and high 1% and 10% concentration of.
These isolates were purified from soil samples on the basis of their colony morphology, texture, growth, temperature and hydrocarbon utilization ability. These bacteria have the ability to catalyze the degradation of hydrocarbons found in the environment. Saturated and aromatic hydrocarbons are widespread in our environment. Hydrocarbon degrading bacteria medina agriculture products. Similarly, in preliminary study 57 oil degrading bacteria were isolated from oil impregnated soil in search of potent hydrocarbon degrader 14. Anaerobic degradation of oil hydrocarbons by sulfate. Pdf identification of hydrocarbon degrading bacteria by. Biodegradation of petroleum hydrocarbons slideshare. Hydrocarbondegrading microorganisms are ubiquitous in the marine environment 27, 76, and.
International journal of current microbiology and applied. Reconstructing metabolic pathways of hydrocarbondegrading. Metabolic diversity of aromatic hydrocarbondegrading. Hydrocarbons, enrichment culture technique, oil degrading bacteria, gravimetric analysis, biochemical tests. Elghazawani2 and ahmed issa3 1microbiology laboratory, botany department, omar almukhtar university, elbeida libya 2inspection and corrosion section, sarir.
Hydrocarbondegrading microorganisms are ubiquitous in the. Interest in possible hydrocarbon oxidation by sulfatereducing bacteria arose from their frequent presence in oil production. Hydrocarbondegrading microorganisms, adapted to grow and thrive in these environments, play an important role in the biological treatment of polluted extreme habitats. Isolation, identification and characterization of oil. Isolation and characterization of hydrocarbon degrading. One potent gram negative bacillus bacteria was isolated on the basis of petroleum hydrocarbon degradation efficiency and identified as pseudomonas sp. The optimization of abiotic and biotic factors should be the focus of future bioremediation of oil contaminated soil. Aerobic bacteria degrading both n alkanes and aromatic. Comparative studies on bacterial consortia for hydrocarbon degradation.
Isolation, identification and characterization of oil degrading bacteria isolated 431 biochemical characterization of selected microorganisms. Pdf characterization of hydrocarbon degrading bacteria. Supplementary tables 1,2, figures 16 and references. Pages in category hydrocarbon degrading bacteria the following 9 pages are in this category, out of 9 total. Figure 2 shows the main principle of aerobic degradation of hydrocarbons. Several bacteria are even known to feed exclusively on hydrocarbons 11. Bacteria and fungi are the key agents of degradation, with bacteria assuming the dominant role in. Studies on crude oil degrading bacteria isolated from. Hydrocarbon degradation and enzyme activities of cold. Isolation and molecular identification of hydrocarbon.
Bayoumi and abulhamd 2010, have reported isolation of one hundred and nine 109 pure bacterial cultures in mineral salt medium msm supplemented with toluene and phenol. Isolation and characterisation of crude oil sludge. Pdf hydrocarbon contamination in the environment has been a notable problem since years. Isolation of hydrocarbon degrading bacteria following the strategy designed in our work to isolate hydrocarbondegrading bacteria both with high growth and biological activity, 39 purified bacterial isolates were recorded amongst hundreds of isolated ones at the first step of the program. Bacterial degradation and bioremediation of polycyclic aromatic hydrocarbons article pdf available in polish journal of environmental studies 121. Adeleke1,3,4 background high demand for petroleum products all over the world has led to an increase in crude. The efficiency of bacterial and yeast strains was compared. Isolation and identification of effective hydrocarbon. While hydrocarbondegrading microorganisms are ubiquitous, hydrocarbon. The goal of this project was to evaluate the effects of test conditions and bacterial growth conditions on the results and precision of the math test using a single species of hydrocarbon degrading bacteria. Native crude oil degrading bacteria were isolated from different crude oil polluted soils.
Emulsify bioactivate digest product description application instructions spill kit 35s contains a plant derived there is no standard protocol for the biosurfactant mix called biosurf, and of contaminated soil. These compounds exhibit low chemical reactivity and for many decades were thought to undergo biodegradation only in the presence of free oxygen. Hydrocarbondegrading bacteria and the bacterial community. Screening and isolation of a hydrocarbon degrading bacteria by enrichment culture technique. Despite its toxicity, a considerable fraction of petroleum oil entering marine systems is eliminated by the hydrocarbondegrading activities of microbial communities, in particular by a recently discovered group of specialists, the hydrocarbonoclastic bacteria hcb. Raeid m m abed, jamal alsabahi, fatema almaqrashi, amal al. Altogen labs has developed active bioremediation technology based on natural oildegrading bacteria for largescale remediation of hydrocarboncontaminated water and soil.
The most rapid and complete degradation of the majority of organic pollutants is brought about under aerobic conditions. Most importantly, hydrocarbonoclastic bacteria will decrease the concentration and toxicity of hydrocarbons in the immediate vicinity of algal cells. Petroleum compounds are categorized into 2 groups aliphatic hydrocarbon e. However, cultures have not been preserved, or attempts to reproduce hydrocarbon oxidation by desulfovibrio strains failed 1, 2. Nitratereducing 17 bacteria couple the oxidation of org. Crude oil and petroleum is a complex mixture of hydrocarbons, which cause widespread environmental contaminants following a spill. Pdf petroleum hydrocarbondegrading bacteria for the. Studies on crude oil degrading bacteria isolated from libyan desert farag m. Vinoth kumar1 1bioprocess engineering laboratory, department of biotechnology, srm university, chennai, india.
Analysis of aromatic hydrocarbon degrading capacity by thermophilic bacteria isolated from oil contaminated soil bitan basu1, ramanathan ramasamy1, sivasubramanian viswanathan1, iyyappan divya priya1, jayarajan jeyan1, amala reddy1, v. Petroleum hydrocarbondegrading bacteria and the type of petroleum components they. Screening for potential hydrocarbon utilizing bacteria by dcpip assay has been reported 4, 7, 9, 14, 15, 20. Medina hydrocarbon degrading bacteria consists of known nonpathogenic microbial strains that degrade petroleum hydrocarbons in solid and aqueous environments. The initial intracellular attack of organic pollutants is an oxidative process and the activation as well as incorporation of oxygen is the enzymatic key reaction catalyzed by oxygenases and peroxidases. Pdf a significant portion of oil from the recent deepwater horizon dh oil spill in the. The bootstrapped neighborjoining phylogenetic tree was generated in mega using the. Crude oil bioremediation of soils is limited by the bacteria activity in degrading the spills hydrocarbons. Hydrocarbonoxidizing bacteria have potential applications in bioremediation of oil pollution, enhanced oil recovery, production of surfaceactive agents, and in the use of hydrocarbons as substrates for industrial fermentation processes. Characterization of hydrocarbondegrading microbial. Isolation and characterisation of crude oil sludge degrading bacteria linda u. Department of chemistry, rochester institute of technology, rochester, ny. Enrichment, isolation and identification of hydrocarbon degrading bacteria udgire m. In the present study, altogether 32 morphologically distinct bacteria were isolated from crude oil contaminated soil and 18 different hydrocarbondegrading bacteria were number.
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